So what i want to do is essentially suck a line of txt from a .txt file, then assign the characters to a list, and then creat a list of all the separate characters in a list.
So a list of lists.
At the moment, I've tried:
fO = open(filename, 'rU')
fL = fO.readlines()
fL = 'FHFF HHXH XXXX HFHX'
['F', 'H', 'F', 'F', 'H' ...]
Strings are iterable (just like a list).
I'm interpreting that you really want something like:
fd = open(filename,'rU') chars =  for line in fd: for c in line: chars.append(c)
fd = open(filename, 'rU') chars =  for line in fd: chars.extend(line)
chars =  with open(filename, 'rU') as fd: map(chars.extend, fd)
chars would contain all of the characters in the file.