Is it possible to use
You can use
calc() wherever you can use a length based value in CSS. The example you have provided does work, but actually adds up to 0. Here is a slightly changed demo to illustrate: http://jsfiddle.net/joshnh/6ydR3/
Also, make sure to list the unprefixed version last.
Mixing percentages with other value types in the calc() function within a transform doesn't seem to work at all in Chrome. I'll report this as a bug.
UPDATE: This has been reported as a bug here: https://code.google.com/p/chromium/issues/detail?id=150054