Basically what I am trying to do is read in each character from each line into a list, and after each line, add that list into another list (one list per line in input file, each list containing all the individual characters of each line)
This is what I have so far but it doesnt seem to be working and I can't figure out why.
allseq = 
with open("input.txt", "r") as ins:
seq = 
for line in ins:
for ch in line:
if ins != "\n":
seq[:] = 
Strings in Python can be easily converted into literal lists of characters! Let's make a function.
def get_char_lists(file): with open(file) as f: return [list(line.strip()) for line in f.readlines()]
This opens a file for reading, reads all the lines, strips off extraneous whitespace, sticks a list of the characters into a list, and returns that last list.